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KMID : 0350519940470041735
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Journal of Catholic Medical College
1994 Volume.47 No. 4 p.1735 ~ p.1747
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Clinical Significance of the Serum Pattern of Different Molecular Forms of Prolactin in Women with Hyperprolactinemia
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Abstract
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Human prolactin(hPRL) has been shown to be structurally heterogeneous with multiple forms of different molecular sizes. Most of these forms have been detected in the pituitary gland and plasma but their exact proportions and physiological
significance
of heterogeneity are not yet fully determined but are probably relate to the polyfunctional action of the hormone.
In this study, the molecular heterogeneity of hPRL was analysed by Western blotting, immunostaining and densitometric readings in serum from 25 cases of hyperprolactinemia patients showing endocrinologic manifestation and 8 cases of normal
pregnant
women. At the same time, the relationship between the proportions of hPRL component and clinical manifestations of the women with hyperprolactinemia were analysed.
@ES The results were as follows:
@EN 1. Only the 25 kilodalton(KD) band was present when Con A-sepharose bound fractions were analysed ; thus we consider it to be the glycosylated hPRL(G-hPRL).
2. In total 33 cases of serum, seven specific hPRL bands were identified with the apparent high molecular weight of 64kD, 53kD, 45kD, the low molecular of 23kD, 21kD, 16kD and 25kD as G-hPRI.
Among them 64kD, 53kD, 25kD and 23kD were identified in all subjects and the others were identified in 5 subjects(15.2%) in 45kD, 22(66.7%) in 21 kD and 18(54.5%) in 16kD.
3. As fol the galactorrhea, increase of the serum hPRL was statistically significant but low/high molecular weigh ratio NG-hPRL/G-hPRL ratio were not significant.
4. As for the irregular menstruation, increase of low/high molecular weight ratio and NG-hPRL/G-hPRL ratio were statistically significnat but the serum level of hPRL was not significant.
5. As for the infertility, increase of low/high molecular weight ratio was statistically significant but the serum level of hPRL and NG-hPRL/G-hPRL ratio were not sigificant.
6. As for the progesterone shortage, increase of low/high molecular weight ratio statistically significant but the serum level of hPRL and NG-hPRL/G-hPRL ratio were not sigificant.
7. As for the pregnancy progress, increase of the serum hPRL was statistically significant but low/high molecular weight ratio and NG-hPRL/G-hPRL ratio were not significant.
From these results, we concluded that the consistent pattern of change in the relative proportions of hPRL component in the serum during specific conditions suggests that these modifications are physiologically important and may be correlated
with
the
bioactivity of hPRL in order to fulfill specific biological needs.
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KEYWORD
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